Journal: Molecular Microbiology

Article Title: Cell envelope defects of different capsule‐null mutants in K1 hypervirulent Klebsiella pneumoniae can affect bacterial pathogenesis

doi: 10.1111/mmi.14447

Figure Lengend Snippet: Intraperitoneal infection of C57BL/6J mice with SGH10, SGH10Δ rmpA and SGH10Δ wcaJ . (a) Bacterial loads in the liver, (b) lungs and (c) spleen were measured by plating at 30 hr post infection. Mean ± SEM are plotted and * denotes p < .05, while ** denotes p < .001

Article Snippet: For all experiments, female C57 BL/6 JAX® mice aged 7–8 weeks were purchased from InVivos.

Techniques: Infection

Journal: Journal of Pharmaceutical Analysis

Article Title: Coral calcium hydride promotes peripheral mitochondrial division and reduces AT-II cells damage in ARDS via activation of the Trx2/Myo19/Drp1 pathway

doi: 10.1016/j.jpha.2024.101039

Figure Lengend Snippet: Molecular hydrogen mediates the activation of Trx2. (A) Representative images of immunohistochemical staining for Trx2 in lung tissue ( n = 3 in each group). (B) The plot of integral optical density (IOD) of Trx2 in lung tissue ( n = 3 in each group). (C) Mitochondria were purified from lung tissue. Representative images of Western blot for Trx2 ( n = 3 in each group) and quantitative analysis. (D) The plot of mean fluorescence intensity of Trx2 and representative images of immunofluorescence staining for Trx2 in lung sections removed at 12 h ( n = 3 in each group). (E) Representative images of immunohistochemical staining for Trx2 in A549 cells ( n = 3 in each group). (F) The plot of IOD of Trx2 in A549 cells ( n = 3 in each group). (G) A549 cell mitochondria were purified. Representative images of Western blot for Trx2 ( n = 3 in each group). (H) The plot of mean fluorescence intensity of Trx2 and representative images of immunofluorescence staining for Trx2 in A549 cells at 12 h ( n = 3 in each group). Data are presented as means ± standard deviation (SD), and were analyzed by one-way analysis of variance (ANOVA) followed by Bonferroni's post hoc test. ∗ P < 0.05 versus control; # P < 0.05 versus lipopolysaccharide (LPS)-induced group. CCH: coral calcium hydrogenation; DAPI: 4',6-diamidino-2-phenylindole.

Article Snippet: Wild-type (C57 BL/6) male mice (age 6–8 weeks) and Trx2 knockout (C57 BL/6) male mice (age 6–8 weeks) were provided by Jiangsu GemPharmatech Co., Ltd (Nanjing, China).

Techniques: Activation Assay, Immunohistochemical staining, Staining, Purification, Western Blot, Fluorescence, Immunofluorescence, Standard Deviation, Control

Journal: Journal of Pharmaceutical Analysis

Article Title: Coral calcium hydride promotes peripheral mitochondrial division and reduces AT-II cells damage in ARDS via activation of the Trx2/Myo19/Drp1 pathway

doi: 10.1016/j.jpha.2024.101039

Figure Lengend Snippet: Coral calcium hydrogenation (CCH) treatment of Trx2 +/− lipopolysaccharide (LPS)-induced acute respiratory distress syndrome (ARDS) mice showed no improvement in acute lung injury. (A) The ultrasound examination images of lung tissue ( n = 3 in each group). (B) The lung function test images of lung tissue ( n = 3 in each group). (C) The Speckle Doppler detection of local pulmonary blood flow images of lung tissue ( n = 3 in each group). (D) Representative images of hematoxylin and eosin (HE)-stained lung tissue sections ( n = 3 in each group). (E) Mitochondrial purification of 12 h lung tissue. The expressions of Trx2, Myo19, and p-Drp1 were performed by Western blotting ( n = 3 in each group). Data are presented as means ± standard deviation (SD), and were analyzed by one-way analysis of variance (ANOVA) followed by Bonferroni's post hoc test. ∗ P < 0.05 versus control; # P < 0.05 versus LPS-induced group. Drp1: dynamin-related protein 1.

Article Snippet: Wild-type (C57 BL/6) male mice (age 6–8 weeks) and Trx2 knockout (C57 BL/6) male mice (age 6–8 weeks) were provided by Jiangsu GemPharmatech Co., Ltd (Nanjing, China).

Techniques: Staining, Purification, Western Blot, Standard Deviation, Control

Journal: Journal of Pharmaceutical Analysis

Article Title: Coral calcium hydride promotes peripheral mitochondrial division and reduces AT-II cells damage in ARDS via activation of the Trx2/Myo19/Drp1 pathway

doi: 10.1016/j.jpha.2024.101039

Figure Lengend Snippet: Changes in Myo19/P-dynamin-related protein 1 (Drp1) in A549 cells were observed by overexpressing and silencing Trx2 . (A) Quantitative polymerase chain reaction (q-PCR) analysis of Trx2 mRNA expression with Trx 2 -containing plasmid transfection in A549 cells ( n = 3 in each group). (B) qRT-PCR analysis of Trx2 mRNA expression with Trx2 knockdown plasmid transfection in A549 cells (n = 3 in each group). (C) Western blot analysis of Trx2 protein expression after transfected with Trx 2 -containing plasmid in A549 cells ( n = 3 in each group). (D) Western blot analysis of Trx2 protein expression after transfecting T rx2 knockdown plasmid in A549 cells ( n = 3 in each group). (E) Representative images of Trx2, Myo19 and p-Drp1 expression detected by Western blotting after overexpression of Trx2 for 12 h ( n = 3 in each group). (F) Representative images of Trx2, Myo19, and p-Drp1 expression detected by Western blotting after knockdown of Trx2 for 12 h ( n = 3 in each group). (G) A549 cells were stained with immunofluorescence at 12 h, showing Trx2, Myo19 and p-Drp1 activation in the hydrogen-treated group. A significant difference was revealed by one-way analysis of variance (ANOVA). ∗ P < 0.05 versus control; # P < 0.05 versus lipopolysaccharide (LPS)-induced group; & P < 0.05 versus LPS-induced hydrogen therapy group. OE: overexpression; Si: small interfering; DAPI: 4',6-diamidino-2-phenylindole.

Article Snippet: Wild-type (C57 BL/6) male mice (age 6–8 weeks) and Trx2 knockout (C57 BL/6) male mice (age 6–8 weeks) were provided by Jiangsu GemPharmatech Co., Ltd (Nanjing, China).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Plasmid Preparation, Transfection, Quantitative RT-PCR, Knockdown, Western Blot, Over Expression, Staining, Immunofluorescence, Activation Assay, Control